A problem in multi-objective dynamic facility layout is achieving distance- and adjacency-based objectives for arranging facility layouts across multiple time periods. As a non-deterministic polynomial time-hard problem, it resembles the quadratic assignment problem (QAP), which can be solved through meta-heuristics such as ant colony optimization (ACO). This study investigates three multi-objective approaches coupled with ACO to solve this problem. As the experimental design, we apply the proposed methods to solve the dynamic facility layout problem (DFLP), multi-objective facility layout problem, and multi-objective DFLP based on data sets from the literature to test the quality of the solution. The results show that the proposed methods are effective for solving the problem.